Partial purification and characterization of the mRNA for rat preproinsulin.

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Preproinsulin mRNA in the rat eye.

PURPOSE The goal of this study was to extend the results of previous immunoassay, immunocytochemistry, and in situ hybridization studies showing the presence of insulin-related peptide in the rat retina by confirming the expression of insulin genes in the rat eye. METHODS Total and poly(A)+ RNA were isolated from whole rat eyes, and separately from the retina, choroid, iris, lens, and vitreou...

متن کامل

Purification and Partial Characterization of Rat Liver Lipoxygenase

Lipoxygenase, Enzyme Purification, Hydrophobic Chromatography Lipoxygenase was purified from rat liver cytosolic fraction by a method involving two successive chromatographic steps on Sephacryl S-200 and Phenyl Sepharose CL-4B. The enzyme has a molecular weight of 96 Kdal and it seems to be composed of two identical subunits. Chromatofocusing of the enzyme revealed a single band of activity at ...

متن کامل

Purification and partial characterization of rat liver bilirubin glucuronoside glucuronosyltransferase.

Bilirubin glucuronoside glucuronosyltransferase (EC 2.4.1.95) converts bilirubin monoglucuronide to bilirubin diglucuronide and is concentrated in plasma membrane-enriched fractions of rat liver homogenates. The enzyme was purified 2,000-fold to homogeneity from rat liver. The pI of the enzyme is 7.9 +/- 0.2. The enzyme has a molecular weight of 160,000 and is an oligomer of 28,000 dalton subun...

متن کامل

Purification and Partial Characterization of Rat Liver Bilirubin

Bilirubin glucuronoside glucuronosyltransferase (EC 2.4.1.95) converts bilirubin monoglucuronide to bilirubin diglucuronide and is concentrated in plasma membrane-enriched fractions of rat liver homogenates. The enzyme was purified 2,000-fold to homogeneity from rat liver. The p1 of the enzyme is 7.9 + 0.2. The enzyme has a molecular weight of 160,000 and is an oligomer of 28,000 dalton subunit...

متن کامل

Partial Purification and Characterization

An enzyme fraction containing phosphatase activity for phosphorylated eukaryotic peptide initiation factor 2 (eIF-2) has been isolated from rabbit reticulocytes and partially characterized, The enzyme efficiently catalyzes release of phosphate from the small subunit of eIF-2 (eIF-2a) that has been phosphorylated by the hemin-controlled repressor. It is shown to restore activity of this phosphor...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1976

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.73.10.3539